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fibrillosum, A good.soleirolioides and you can An effective.polyphyllum, considering the changes found in new 2C DNA items in these types of variety, courtesy its stores for 2 ages when you look at the silica (select over). Ploidy profile and you will 1Cx-philosophy was basically inferred for all almost every other subclades analysed (Table 4 and you can Contour 3).
Particularly, compared with the tiny 1Cx-values regarding An excellent
A described phylogenetic forest of your Asplenium monanthes complex and you may related lineages [just like the displayed for the 30] using inferred ploidy accounts (according to had written chromosome analysis), suggest holoploid genome dimensions (1C-value; grey taverns), and you can inferred monoploid genome dimensions (1C x -value; black taverns) and mean spore size (?m) each and every functional product. Rear part service >0.8 try revealed. Getting diploids only black colored pubs exists because the 1C-well worth is equivalent to the newest 1Cx-well worth. Standard error taverns are included for 1C-worth and you may 1Cx-well worth function, with the exception of lineages depicted by you to sample simply. Forest is actually rooted based on Dyer ainsi que al. . Details of the package plots getting spore proportions are given in the new legend to figure 1.
During the An effective.resiliens clade, more ploidy account was indeed inferred on a couple Good.aff.heterochroum specimens. You to sample from A great.aff.heterochroum (RD75; 2C = pg) got an identical 2C-really worth to A beneficial.resiliens (mean 2C = pg), a variety said to be triploid, indicating you to definitely RD75 may be a triploid. In contrast, the other A.aff.heterochroum sample analysed (RD9a) got a dramatically large 2C-really worth ( pg) and you may a 1Cx-worthy of up to 4 times that inferred having RD75, and therefore resulted in inference that the specimen is actually good tetraploid.
The new idea that most A.monanthes specimens try triploid is dependent on this new observation you to, local hookup in Memphis with the exception of you to statement of a tetraploid amount, the counts for it varieties were 2n = 3x = 108. Not as much as which assumption, the fresh mean 1Cx-philosophy getting MO1 sandwich-clades I, II and you may III is pg, 9.33 g, and 8.95 pg, correspondingly (Table 4). A triploid level is additionally believed to possess MO2 (I), in line with the 2C-thinking, ultimately causing an indicate 1Cx-value of 9.02 pg. The new A great.monanthes MO2 (II) sub-clade, and this comprised just one specimen (RD96), got a substantially straight down 2C-value of pg, so we infer that this sample can be an effective diploid cytotype. This will result in a good 1Cx value of nine.30 pg, that is for the greater agreement towards monoploid genome versions calculated for the kept A.monanthes sandwich-clades (Table cuatro).
Within the A good.hallbergii, the new similarity of its indicate 2C-worthy of ( pg) and you can imply spore dimensions ( ?m) to that of triploid A good.monanthes lineages (MO1 and you will MO2.I) implies this varieties is additionally good triploid. When it comes to a few the latest kinds, specification.the fall of.step one and specification.the fall of.2, they disagree into the 2C-thinking ( pg and you can pg correspondingly) but have similarly small spore versions. He or she is currently inferred to-be diploid even when then investigation try had a need to confirm or refuse which.
Nonetheless, it is listed that suggest spore size of RD96 ( ?m) issues using this type of looking, suggesting this particular sample are a good polyploid
Total, the outcomes show that monoploid genome proportions varies 2.4-flex (cuatro.66- pg), with little to no adaptation in 1Cx-philosophy within this clades, however, big type ranging from some of them (Desk cuatro and you may Shape step three). resiliens clade (4.66-cuatro.79 pg), the newest mean 1Cx-value for diploid An excellent.formosum (6.81 pg) is markedly higher. More substantial monoploid genome proportions has also been listed throughout the Good.monanthes clade compared to the newest Good.resiliens clade. Indeed, the biggest monoploid genome sizes was indeed came across in A great.monanthes clade (8.70- pg), which have specification.the fall of.2 having the high 1Cx-worth ( pg) of all, that’s over twice as much imply monoploid genome size of this new A.resiliens clade.